2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. Example: [Drawer]. Sale. The SpectraMax® iD3 Multi Mode Microplate . Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. Also see:. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. edu) to be added as a trained user in iLab. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. . 2. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. Cuvette Microplate wells. SpectraMax® Microplate Readers and SoftMax® Pro Software. These robust readers have been placed in labs from. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. We would like to show you a description here but the site won’t allow us. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 3) This warranty. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 2. The dual monochromator optics allow. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 020 fmol 0. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Microplate Readers. Create custom fluorescence assays without having to purchase expensive filter sets. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. 0 out of 5 $ 2,999-20%. Cell Cytotoxicity assay procedure. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Offer Ends In: 2 Days 10: 40: 19. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). SpectraDrop High-Throughput Screening (HTS) Kit. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. We would like to show you a description here but the site won’t allow us. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 1 software Download page. Instrument settings used with the SpectraMax M5 Microplate Reader. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. Fully tested with validations, warranty. All . Figure 1. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 0 out of 5 $ 5,999. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. 3) At the bottom of the strip, click on "Show. SpectraMax Spectrofluorometers. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. 0 out of 5 $ 23,995 $ 22,495. flat meniscus), but is approx 15 mL. View the Molecular Devices website. Summary of Contents for Molecular Devices SpectraMax M2. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Highly customizable low volume. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2 only: SpectraMax Mini Update. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. SpectraMax ® M2 Multi-Mode Microplate Reader. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Results are shown in Table 1. Temperature control. Using the. In . We use Spectramax M5 microplate reader in our lab. limit of detection (LLD) for ATP was estimated for each microplate reader using the . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. Figure 3. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Molecular Devices Spectramax iD3 Microplate Reader. Our specialization servicing. 1. 305 reference level saturation. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. 0 out of 5 $ 5,999. Italic and boldface type are used for emphasis. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. The guide also covers the software features, the specifications, and the accessories of the systems. keeping with best practices, reader performance should. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. 40266 Email: drugdiscoverytech@lifetech. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. When the user applies a set temperature of 37°C, the top is heated to 37. The screw remains inside the instrument. 7°C and the bottom to 36. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax M5 reader settings are shown. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Total volume (except deep. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The University of Rouen in Normandy uses the SpectraMax iD3 and. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 306 plate air cal. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Absorbance (optical density, OD) was read at 570 nm and 605. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. . SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Download PDF versions of the SpectraMax Microplate Reader User Guides. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 305 reference level saturation. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Detailed specifications of each model reader are reported in Section. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. begin reading microplates with seven mouse clicks. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. Microplate Readers BioTek Epoch 2 Microplate Reader. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Download PDF. It also integrates with the. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. For a higher . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The plate reader optics are tunable for. HTRF-compatible SpectraMax Multi-Mode Readers. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Type:Spectrophotometer. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Thank you!. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax 250 User. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. both cuvette-based and microplate reader applications on the same instrument. 7°C (gradient heating design). . Our absorbance plate readers feature our PathCheck Sensor technology and. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. FI assay. Tecan Infinite Microplate Reader. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. . This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. A: Microplate Preparation. both cuvette-based and microplate reader applications Microplate Readers. For readers without a cuvette port, these options are not shown in the software. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Published Dec 16, 2020 | Updated May 19, 2023. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Molecular Devices SpectraMax M2E Microplate Reader. Or, with the reader specifications noted from the Instrument Description section of your reader's. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Create custom fluorescence assays without having to purchase expensive filter sets. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. Category 2 and 3 microplate readers use gradient heating as well. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. 100 fmol 384-well (75 µL) 0. 100 readings per well gives optimal Z’ values for this assay. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Results are shown in Table 1. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Multimode Microplate Readers. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Microplate reader settings. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Cell Cytotoxicity assay procedure. Microplate reader assays requiring infrared (IR) or red-shifted. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Unparalleled performance on a personalized platform. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 0 out of 5 $ 5,999. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The SpectraMax® ABS Plus Microplate Reader can run . Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. 58% for the detection module and 2. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Endpoint, kinetic, spectrum. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Supplier: Molecular Devices. Request a Quote!Introduction. 1. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. Access this by going to Help >. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Page 28 Chapter 5: Read Modes and Read Types 1. flexibility of monochromator-based systems. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. . SpectraMax i3x Multi-Mode Detection Platform. begin reading microplates with seven mouse clicks. The dual monochromator optics allow the. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. no. Table of Contents. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 4 μL DNA standards. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. e. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 470 nm). The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. View the Molecular Devices website. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. These lab workhorses are used in drug discovery. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. In addition, the SpectraMax® iD5 can be expanded to include bottom. Maximum signal with lowest background. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Find regional support. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Temperature control. B: Selection of Excitation Wavelength. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Prerequisites: SoftMax Pro 7. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. On the back, in the right hand top corner,. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Example: [Drawer]. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. These lab workhorses are used in drug discovery, research, bioassay. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Fluorescence Microplate Readers. Assays vary depending on. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 0200-6263. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. More Product Information. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. This unlocks the transport slide. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). We would like to show you a description here but the site won’t allow us. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Transcreener ® ADP. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. Microplate Readers. Multimode Microplate Reader. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. . We would like to show you a description here but the site won’t allow us. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Path Check Pathlength Measurement every Gemini EM Reader. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Dual-Glo luciferase assay system (Promega Cat. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. microplates or in a cuvette at a. 997 (n = 3). The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. The drawing shows a standard microplate of 15 mm height covered with a lid. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. 2. 1. 2. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. no. The instrument uses a paired monochromator and xenon flash. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength.